The Architecture of Oligonucleotide‐Polycationic Brush Complexes – A Neutron Reflectometry Study

Polycationic brushes are attractive systems for the design of nanomaterials gene delivery as they enable rational their architecture with a broad range grafting densities, thicknesses, and chemistries. Recently, performance siRNA is highlighted strong impact molecular on RNA binding transfection efficiency now calls greater understanding polymer brush-oligonucleotide complexes. In this study, morphology chemistry (weak polybase poly(dimethylaminoethyl methacrylate), PDMAEMA, poly(2-methacrylolyloxyethyltrimethylammonium iodide), PMETAI) complexes 20 base pair oligonucleotides first investigated. These assemblies then studied via neutron reflectometry, building model brush swelling in deionized water, at three different contrasts, prior to investigation brush-RNA It found that infiltrate deep within alter most basal layer more strongly, regardless strength polybase. This will improved nanostructures applications.